cloning and expression of hepatitis b surface antigen
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چکیده
منابع مشابه
EXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN SACCHAROMYCES CEREVISIAE
The genome of HB V virus of serotype ayw cloned in pBR322 and expression shuttle vector p YES2 were used for construction of the HBsAg chimeric genes and their expression in Saccharomyces cerevisiae. Two recombinant plasmids were constructed. One of them contained the coding sequences for the major polypeptide of surface antigen. Another construct carried the major polypeptide with the pre-S2 a...
متن کاملConstruction and Expression of Hepatitis B Surface Antigen Escape Variants within the "a" Determinant by Site Directed Mutagenesis
Background: The antibody response to hepatitis B surface antigen (HBsAg) controls hepatitis B virus infection. The "a" determinant of HBsAg is the most important target for protective antibody response, diagnosis and immunoprophylaxis. Mutations in this area may induce immune escape mutants and affect the performance of HBsAg assays. Objectives: To construct clinically relevant recombinant muta...
متن کاملexpression of hepatitis b surface antigen in saccharomyces cerevisiae
the genome of hb v virus of serotype ayw cloned in pbr322 and expression shuttle vector p yes2 were used for construction of the hbsag chimeric genes and their expression in saccharomyces cerevisiae. two recombinant plasmids were constructed. one of them contained the coding sequences for the major polypeptide of surface antigen. another construct carried the major polypeptide with the pre-s2 a...
متن کاملExpression of hepatitis B surface antigen in transgenic plants.
Tobacco plants were genetically transformed with the gene encoding hepatitis B surface antigen (HBsAg) linked to a nominally constitutive promoter. Enzyme-linked immunoassays using a monoclonal antibody directed against human serum-derived HBsAg revealed the presence of HBsAg in extracts of transformed leaves at levels that correlated with mRNA abundance. This suggests that there were no major ...
متن کاملExpression of hepatitis B surface antigen gene in yeast.
The DNA sequence coding for hepatitis B virus surface antigen (HBsAg) was placed under control of the repressible acid phosphatase promoter of the yeast Saccharomyces cerevisiae in a plasmid capable of autonomous replication in both yeast and Escherichia coli. Yeast transformed by this plasmid synthesized up to 5 X 10(5) molecules per cell of immunologically active HBsAg polypeptide in phosphat...
متن کاملHigh-level Expression of Hepatitis B Surface Antigen In
............................................................................................................... iv ÖZ.................................................................................................vi ACKNOWLEDGEMENT ........................................................................................... ix TABLE OF CONTENTS ......................................................
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عنوان ژورنال:
hepatitis monthlyجلد ۸، شماره ۱، صفحات ۱۷-۲۱
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